Description
For production of protein from target genes cloned in pET vectors by induction with IPTG
Description
- B834 is parental strain for BL21 (1)
- Protease-deficient hosts are methionine auxotrophs
- Allows high specific-activity labeling of target proteins with methionine and selenomethionine for crystallography
- pLysS strains express T7 lysozyme
- Suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability
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